Human tandem repeat sequences in forensic DNA typing
نویسندگان
چکیده
منابع مشابه
Human tandem repeat sequences in forensic DNA typing.
It has been 20 years since the first development of DNA fingerprinting and the start of forensic DNA typing. Ever since, human tandem repeat DNA sequences have been the main targets for forensic DNA analysis. These repeat sequences are classified into minisatellites (or VNTRs) and microsatellites (or STRs). In this brief review, we discuss the historical and current forensic applications of suc...
متن کاملShort tandem repeat typing technologies used in human identity testing.
Short tandem repeat (STR) typing methods are widely used today for human identity testing applications including forensic DNA analysis. Following multiplex PCR amplification, DNA samples containing the length-variant STR alleles are typically separated by capillary electrophoresis and genotyped by comparison to an allelic ladder supplied with a commercial kit. This article offers a brief perspe...
متن کاملShort tandem repeat DNA typing provides an international reference standard for authentication of human cell lines.
Cell lines have wide applications as model systems in the medical and pharmaceutical industry. Much drug and chemical testing is now first carried out exhaustively on in vitro systems, reducing the need for complicated and invasive animal experiments. The basis for any research, development or production program involving cell lines is the choice of an authentic cell line. Microsatellites in th...
متن کاملIntegrated portable polymerase chain reaction-capillary electrophoresis microsystem for rapid forensic short tandem repeat typing.
A portable forensic genetic analysis system consisting of a microfluidic device for amplification and separation of short tandem repeat (STR) fragments as well as an instrument for chip operation and four-color fluorescence detection has been developed. The microdevice performs polymerase chain reaction (PCR) in a 160-nL chamber and capillary electrophoresis (CE) in a 7-cm-long separation chann...
متن کاملMicrochip Analyzer for Forensic Short Tandem Repeat Typing of Single Cells
Short tandem repeat (STR) typing at the single-cell level is a promising tool for human forensic identification when the biological evidence materials are comprised of mixtures of cells from multiple individuals at relatively low concentrations. Here we describe a novel single-cell STR typing method with high sensitivity, fidelity and throughput that combines microfluidic droplet generation wit...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Legal Medicine
سال: 2005
ISSN: 1344-6223
DOI: 10.1016/j.legalmed.2005.02.002